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Fig. 7. Induction of stalk cell differentiation by DIF-1. Comparison of the DIF-1
sensitivity of AX2 cells and an Ax2/gskA– strain. Ax2 (black circles)
and gskA-null mutant cells (white circles) were plated at low density
(104 cells/cm2) in stalk medium in 5 mM camp). After 24
hours incubation, cAMP was washed off and replaced with DIF at varying
concentrations for a further 24 hours. Stalk cells were counted and expressed
as a percentage of total number plated. Data are expressed as
mean±s.e.m., and represent the combination of two clones analysed in
triplicate. (B) Comparison of the effect of cAMP on AX2 cells, a random
integrant and an Ax2/gskA– strain. Cells were treated as in A, but
incubated in 100 nM DIF±5 mM cAMP, as indicated. (C) Comparison of the
effect of cAMP on AX2 cells and an Ax2/gskA– strain using different
inductive concentrations of DIF-1 Cells were treated as in A, but incubated in
100, 10 or 5 nM DIF ±5 mM cAMP, as indicated.
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