|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 4. Nephrogenic mesenchyme is disorganized and prematurely depleted in
Bmp7cre/+;Smad4–/CA kidneys. Comparison
of wild-type (WT; A,C,E,G,I,K,M,O) and
Bmp7cre/+;Smad4–/CA (Mut;
B,D,F,H,J,L,N,P) kidneys analyzed by section in situ hybridization at E14.5
(A-L) and E16.5 (M-P). (A'-H') High magnification images of
cortical regions. Ret (B) and Pax2 (D) expression is
maintained in the collecting ducts of mutant kidneys indicating normal
morphogenesis of this structure. Pax2 (D) and Wt1 (F,J),
markers of nephrogenic mesenchyme, reveal a disorganized distribution of cells
in the expanded cortical region of mutant kidneys (red brackets), with
occasional clusters of cells surrounding tips of collecting ducts.
Raldh2, a marker of peripheral stroma, identifies cells with
characteristics of peripheral stroma in the expanded cortical region of mutant
kidneys (H,L). Glomerular podocytes are marked by Wt1 in mutant
kidneys (F). At E16.5, Wt1 is absent from the cortical region of the
mutant kidney (bracket in N), but Raldh2 remains (P), suggesting that
the composition of this cell population mirrors that of wild-type peripheral
stroma. CD, collecting duct; G, glomerulus; NM, nephrogenic mesenchyme; PS,
peripheral stroma.
|
