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Fig. 5. Nephrogenic mesenchyme and stroma are incompletely segregated in
Bmp7cre/+;Smad4–/CA kidneys. Comparison
of wild-type (WT; A,C,E,G,I,K,M,O,Q) and
Bmp7cre/+;Smad–/CA (Mut;
B,D,F,H,J,L,N,P,R) at E11.5 (A-H), E12.5 (I-P) and E13.0 (Q,R). At E11.5,
expression of Wt1 (B) and Pax2 (D) in mutant kidneys is
indistinguishable from that of wild type (A,C), indicating that the
metanephric blastema is appropriately patterned. Raldh2 (F) reveals a
distribution of stromal cells in mutants that is comparable to wild type (E).
Expression of Lhx1 (H) at E11.5 indicates that maturing structures
such as renal vesicles appear at approximately the same time in mutant kidneys
as they do in wild type (G). At E12.5, expression of Wt1 (J) reveals
disorganization of the nephrogenic mesenchyme that normally forms around
collecting duct tips. Expression of Wt1 is widespread in the
wild-type mesenchyme at E12.5 (I), and Wt1-negative peripheral stroma
can only be seen in occasional sections. Pax2 expression clearly
demarcates nephrogenic mesenchyme and nascent epithelia from stromal cells in
the wild type (K). Mutant kidneys show a paucity of nephrogenic mesenchyme and
no clear demarcation of stromal cells (L). Raldh2 expression is
confined to the stromal cell compartment of the wild-type kidney (M), and is
excluded from nephrogenic mesenchyme and collecting ducts. In the mutant (N),
Raldh2 expression is spread throughout the majority of the
mesenchyme, and is excluded from the limited nephrogenic mesenchyme population
and collecting ducts. Lhx1 expression reveals several renal vesicles
per section in the wild type (O), but only a limited number in the mutant (P).
At E13.0, Gdnf expression is comparable in the wild type (Q) and the mutant
(R). CD, collecting duct; G, glomerulus; Mes, metanephric mesenchyme; NM,
nephrogenic mesenchyme; PS, peripheral stroma; RV, renal vesicle; UB, ureteric
bud.
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