Supplemental Figure 1
-
Fig.
S1. DiI labeling of
the optic nerve. (A) DiI labeling of an intact E11 eye shows the optic nerve
(on) exiting the eye. (B-D) E11 regenerating eye labeled with DiI. (B) DiI
labeling overlayed on a DIC image of the regenerating eye shows that nerve fibers
exit the regenerating eye (arrows) but do not form an optic nerve. (C) DiI
fluorescence of the region seen in B, demonstrates that the fibers that exit
the eye (arrows) do not exhibit any organization. (D) Close up view of the
boxed region in C, shows a nerve fiber (arrow) that has migrated away from the
eye. S, sclera; l, lens; r, retina; cr, regeneration from CB/CMZ.
