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Fig. 2. Cells in the CB/CMZ are a source of regenerating retina. (A) Collagen type
IX immunohistochemistry performed on an E4 operated eye 4 hours
post-retinectomy shows the CB/CMZ region has not been removed. (B) An adjacent
section shows that the CB/CMZ is rich in cells that express Pax6 (blue) and
Chx10 (red). White arrowheads indicate progenitor cells that co-express Pax6
and Chx10. (C) Another adjacent section shows that within 4 hours of
retinectomy, most cells in the CB/CMZ are proliferating and labeled with BrdU,
suggesting that progenitor cells (like those shown in B) are proliferating.
(D) The CB/CMZ was labeled with DiI (red) immediately after retinectomy. The
collagen type IX (green) immunofluorescence confirms that the labeled region
is the CB/CMZ. (E) At E5, 1 day after the CB/CMZ was labeled, DiI-labeled
cells have migrated from the CB/CMZ and generated a neuroepithelium. (F) Three
days after retinectomy this phenomenon is still apparent. The asterisk
indicates the region where the CB/CMZ was originally labeled with DiI. c,
cornea; CB/CMZ, ciliary body/ciliary marginal zone; l, lens; RPE, retina
pigmented epithelium; pe, pigmented epithelium; cr, retina that regenerated
from the CB/CMZ. The red arrowheads indicate the site at which the incision
was made during the retinectomy. Scale bar in A is for A-C.
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