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Fig. 3. Hypoxia influences the kinetics of mesoderm differentiation. Flow cytometry was performed on dissociated cells obtained from EBs harvested at the indicated time points. (A) The number of FLK1+ cells detected in differentiated Arnt+/+ EBs is increased by hypoxic conditions. In direct contrast, the number of FLK1+ cells in two independent Arnt–/– cell lines is reduced in normoxia and is not enhanced by 3% O2. (B) Hypoxia stimulates the production of GFP+ cells in GFP-BRY ES cells as early as day 2 of differentiation.





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