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Fig. 5. VEGF is not necessary for FLK1+ cell production, but is
necessary for the generation of BL-CFCs. (A) Arnt+/+,
Arnt–/–, Vegf+/– and
Vegf–/– ES clones were differentiated for 3 days
under normoxic (21% O2) or hypoxic (3% O2) conditions
and replated for their hemangioblast potential. Error bars represent SEMs of
triplicate cultures. (B) Generation of BL-CFCs in Arnt+/+
cultures is inducible with the addition of VEGF (5 and 10 ng/ml) during early
time points (day 1.5-2.5), but exogenous VEGF does not rescue the
Arnt–/– cultures. (C) VEGF and bFGF do not
rescue FLK1+ cell production in
Arnt–/– EB cultures;
Vegf+/– and Vegf–/– cells
exhibit no FLK1+ deficiency and FLK1+ cell numbers can
be further induced with the addition of VEGF and bFGF, or by hypoxia
treatment.
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