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Fig. 1. Advent of the spleno-pancreatic mesenchyme and the relationship to the SMP.
(A) Reconstruction of an E10.5 embryo following analysis by optical projection
tomography (OPT). Yellow and red boxes indicate the planes of the sections
shown in B and C. (B,C) Cross-sections through an E10.5 embryo in the region
of the stomach (B) and dorsal pancreas (C). Boxed region in C indicates the
embryonic position of the images shown in D-G, and of comparable images
presented throughout the manuscript. (D-G) Expression of early spleen marker
genes in transverse sections through the dorsal pancreatic bud in E10.5
embryos. In situ hybridisation showing expression of Hox11 (D) and
Nkx2.5 (E) (seen as red signal) in combination with pancreatic marker
anti-PDX1 antibody (green). The double label highlights the relationship of
spleen and pancreas location in the early mesenchyme. Expression of
Wt1 (F) and capsulin/Pod1 (G) (single label shown as white
signal) overlaps Hox11 expression domain and confirms that the dorsal
mesenchyme is the region of the spleen rudiment. There is close association
with the thickened mesodermal layer that does not express the spleen markers
at a high level. The approximate position of the embryonic midline is
indicated by the broken line in D. The thickened epithelium that surrounds the
mesenchyme is outline by the broken lines in D and E. br, branchial arches;
dp, dorsal pancreatic bud; e, eye; fb, forebrain; fl, forelimb; h, heart; hl,
hindlimb; st, stomach. Scale bars: 150 µm.
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