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Fig. 4. Genetic control of SMP formation. (A-C) Analysis of SMP formation in the
asplenic mutants Dh and Bapx1–/–,
using PDX1 antibody (red) and phalloidin (green) at E10. (A) Wild-type embryos
show the characteristic triangular shape of the SMP and spleno-pancreatic
mesenchyme, whereas in Bapx1–/– embryos (B),
although the mesenchyme grows to the left, the triangular shape is compromised
and the pancreatic bud remains near the embryonic midline (broken line). In
the Dh/Dh embryos (C), the SMP is lacking and no obvious leftward
growth is detected. At E10.5 in Bapx1–/–
embryos (E), lateral growth occurs but to a lesser extent than in wild-type
embryos (D), and the pancreas remains at the midline. (F,G) Analysis of SMP
formation in Fgf10–/– embryos following
staining for PDX1 (red), and with propidium iodide (green). Development of the
pancreatic endoderm is severely compromised in the
Fgf10–/– embryos, as revealed by an apparent
loss of PDX1 staining in the mutant embryos (G). Despite this, leftward growth
of the spleno-pancreatic mesenchyme and SMP occurs normally (G).
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