|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 1. Dynactin is required to position photoreceptor cell bodies and nuclei in
the developing third instar eye disc. Photoreceptor cell membranes are stained
with anti-Chaoptin in A-G. (A) In wild type, photoreceptor cell bodies (as
defined in this figure by the position of the nucleus) are precisely arranged
in clusters in the apical region of the eye disc and project axons through the
optic stalk (os) into the brain's optic lobe. (B) In
Glued1 mutants, many photoreceptor neuron cell bodies
leave the apical region of the eye disc (arrowheads) and travel into the optic
stalk (bracket) and brain (arrow). (C) Heterozygous
cpbM143 animals have a wild-type photoreceptor axon
projection pattern, with photoreceptor cell bodies positioned in the eye disc.
(D) In heterozygous cpbM143 animals with homozygous
cpbM143/cpbM143 patches in the visual system,
many photoreceptor cell bodies leave the eye disc and enter the brain
(arrows). (E) An independently generated cpb allele,
cpbF44, also causes photoreceptor cell bodies to enter the
brain (arrows) in eye clones. (F) Photoreceptor mispositioning (arrows) is
also observed in cpbM143/Df(2L)E.2 animals rescued from
early lethality by expression of Cpb from a genomic transgene [pYES-ß]
(see text for details). (G) cpbM143/Df(2L)E.2 animals
rescued by ubiquitous expression of a wild-type Cpb cDNA have normal
photoreceptor positioning. Photoreceptor nuclei are stained with anti-Elav in
H-J. (H) In wild type, photoreceptor nuclei remain in the eye disc and do not
enter the optic stalk. (I) Photoreceptor nuclei are mispositioned in
cpbM143 mosaic eye discs, with patches of eye tissue
missing nuclei (arrows) and Elav-staining nuclei found in the optic stalk
(arrowheads). (J) cpbM143/Df(2L)E.2 animals rescued by
ubiquitous expression of a Cpb cDNA have normal photoreceptor nuclear
positioning.
|
