|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 5. Gene expression profiles at the late gastrula stage are more uniform. (A)
Animal, vegetal, DMZ and VMZ cells were isolated from stage 12 embryos and
analyzed by RT-PCR and dot blot. (B) The gene expression profile of each cell
is shown. In this figure cells with the same expression pattern have been
grouped together rather than being shown in order of their position in a
96-well plate. (C) Gsc in situ hybridization on bisected embryos at
stage 11.5 (parasagittal section, dorsal towards left) and stage 13 (sagittal
section, anterior towards right, dorsal towards the top). At stage 11.5, Gsc
is not detected in ectoderm by in situ hybridization, but by stage 13 a domain
of Gsc expression can be seen in the neurectoderm (ec; arrow) overlying Gsc
expression in the axial mesoderm (m).
|
