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Fig. 4. seu-3 displays hallmark auxin response phenotypes. (A) seu-3 is shorter than wild type with increased shoot branching. (B) The three seu-3 seedlings (right) produce fewer lateral roots than the three wild-type seedlings (left). (C) seu-3 roots are longer than wild-type roots when grown on media containing 100 nM NAA. This indicates that seu-3 is not as sensitive to the inhibitory effects of exogenous auxin on root elongation. (D) Quantification of differences in primary root length in seedlings grown on media lacking hormone relative to seedlings grown on media containing NAA (n≥24). *P<0.01 (t-test). (E,F) Close-up of seedling apices from C. Compare epinastic cotyledons of wild type (E) with more upright cotyledons of seu-3 (F). (G) Angle of root tips in light grown seedlings, 24 hours after rotating the plates 90°. Length of each line represents the number of seedlings with root tips growing at that angle, in 12 30° categories (n=52). seu-3 roots reorient to the gravity vector less successfully than do wild-type roots [P<1*10-4 (t-test)]. Genotypes described are in Col. Scale bars: 1 cm.





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