spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 5. Physical and functional interactions of Frodo and TCF3. (A) Dose-dependent effects of Frodo and Dapper RNA on the activation of pSiaLuc reporter by Dsh. (B) Levels of non-phosphorylated ß-catenin are not changed in stage 10 embryos injected with FrdMO and DprMO in all blastomeres at the eight-cell stage. (C) Frodo constructs used for transfections and microinjections. (D) The association of GST-TCF3 with HA-Frodo and HA-Frd337, but not HA-Frd186, in COS7 cells, revealed in a GST pull-down assay. (E) TCF3 associates with Frodo in Xenopus gastrulae. Four- to eight-cell stage embryos were injected with 200 pg of HA-TCF3 RNA and 1 ng of Flag-Frd337 RNA. Protein complexes were immunoprecipitated with anti-Flag antibodies from gastrula stage lysates. (F) The activation of the pSiaLuc reporter by TCF3-VP16 is suppressed by HA-Frd337, but not by HA-Frd186.





Right arrow Return to article