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Fig. 1. Blocking ß1 integrin induces formation of an ectopic joint. Forelimbs
of E14.5 mouse embryos cultured for 4 days were injected at the wrist with 10
µg of an irrelevant IgG (A-D) or with 10 µg of an anti-ß1 integrin
mAb (E-H). (A,E) Complete limb stained with Alcian Blue/Alizarin Red to show
skeletal elements. Inset shows the area in the red square. (B,F) Histological
section of zeugopod region stained with Safranin O and Fast Green to show
cartilage in red. (C,G) In-situ hybridizations for Wnt14. (D,H)
In-situ hybridizations for Gdf5. (I) Histological sections of
zeugopod region in limbs injected with 2 µg RGE peptide stained with
Safranin O and Fast Green. (J) Histological sections of zeugopod region in
limbs injected with 2 µg RGD peptide stained with Safranin O and Fast
Green. (K,L) TUNEL assay for apoptosis, forelimbs injected at the wrist with
10 µg of an irrelevant IgG (K) or with 10 µg of an anti-ß1 integrin
mAb (L).
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