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Fig. 2. Blocking 
5ß1 integrin induces molecular changes characteristic
of joint formation. Forelimbs of E14.5 mouse embryos cultured for 4 days were
injected at the wrist with 10 µg of an anti-5 integrin mAb
(A-G,I,K,M,O). Control injected with 10 µg of an irrelevant IgG (H,J,L,N).
(A) Histological sections of zeugopod region stained with Safranin O and Fast
Green to show cartilage in red. In-situ hybridizations for Wnt14 (B),
Gdf5 (C), chordin (D), autotaxin (E). Immunofluorescence for CD44
(F), type III collagen (G), type II collagen (H-I), type I collagen (J-K),
type X collagen (L-M) and Indian hedgehog (N-O). Immunofluorescence staining
for IHH and for type I, II, III and X collagen is green; cell nuclei were
stained with propidium iodide (red).
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