|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 2. (A) Map of the Fab-7 boundary and the various HS1 subfragments
used in the transgene assays. The virilis homology region is
indicated by V. (B) Map of the ftz:hsp70-lacZ reporter
construct. Inserts placed upstream of the ftz UPS enhancer are in the
non-blocking position (NB), while inserts placed between the NE enhancer and
the hsp70 promoter are in the blocking position. (C,D) lacZ
expression in embryos from representative lines homozygous for
ftz:hsp70-lacZ transgenes carrying different boundary elements. (C)
Random DNA, five binding sites for Su(Hw) and 1.2kb Fab-7. (D)
Tetramerized pHS1 fragments: pHS1x4 in the blocking position, pHS1x4 in the
non-blocking position (NB), pHS1Ax4 and pHS1Bx4. All embryos in Fig. 2 (and
also in Fig. 4) were stained in
parallel as described elsewhere (Hagstrom
et al., 1996) for a direct comparison of staining intensities.
|
