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Fig. 2. Embryonic expression pattern of Sp8 in chick, mouse and zebrafish.
(A-M) Chick embryos showing expression of Sp8 from dorsal views
(A-E,H-J,M) with the anterior towards the top, a lateral view (G), and
transverse sections (F,K,L). (N-R) Mouse embryos showing expression of
Sp8 from lateral views (N-Q) and a dorsal view (R). (S-Y) Zebrafish
embryos showing sp8 expression from lateral views (S-V,X,Y) and a
dorsal view (W) with the anterior towards the left. Pre- and
early-somitogenesis stage expression of chick Sp8 was detected at HH
stage 5 (A), stage 7 (B), stage 8 (C) and stage 9 (D). (E) Sp8
expression in the surface ectoderm of the limb-forming field (arrowheads) at
HH stage 15. (F) A 30 µm section of the embryo in (E) at the forelimb
level, showing the expression in the surface ectoderm (arrowheads) and in the
neural tube. (G) Head region of the embryo in E showing the expression in the
MHB. Sp8 expression in the surface ectoderm in the forelimb field at
HH stage 16 (H) and stage 17 (I). (J) Sp8 expression in the AER at HH
stage 21. In situ hybridization on 14 µm sections at the forelimb level of
a HH stage 21 embryo shows a signal in the AER and the surface ectoderm (K)
and signal in the proliferating interneuron (L). Sp8 expression in
the forelimb AER at HH stage 28 (M). Mouse Sp8 expression at E8.75
(N) and E9.5 (O). (P,Q) The signal in the forelimb-forming region was detected
in the ventral ectoderm at E9.5. The embryonic stage in P is slightly earlier
than that of Q. (R) Strong Sp8 expression in the AER at E10.5.
Zebrafish sp8 expression at 12 hpf (S), 16 hpf (T) and 20 hpf (U).
sp8 expression in pectoral fin buds (arrowhead) at 36 hpf (V) and 48
hpf (W). The expression in the fin bud was observed in the apical fold at 36
hpf (X) and 48 hpf (Y). d, dorsal side; f, forebrain; fl, forelimb; mhb,
midbrain/hindbrain boundary; n, Hensen's node; tb, tail bud; v, ventral
side.
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