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Fig. 3. Embryonic expression pattern of Sp9 in chick, mouse and zebrafish. (A-I) Chick embryos showing expression of Sp9 from dorsal views (A-C,E,H,I); a lateral view (D) with the anterior towards the top; a frontal view (F) with the dorsal towards the top; and a transverse section (G). (J-L) Mouse embryos showing expression of Sp9 from dorsal views. (M-S) Zebrafish embryos showing sp9 expression from lateral views (M-O,Q,R) and a dorsal view (P) with the anterior towards the left, and a transverse section (S). Chick Sp9 expression at HH stage 8 (A), stage 9 (B), stage 10 (C) and stage 13 (D). (E,F) Sp9 expression in the AER at HH stage 21. (G) Transverse section of the limb in E shows the signal in the AER and surface ectoderm. (H) Sp9 is expressed in a small area in the anterior margin (arrow) in addition to the AER at HH stage 27. Sp9 expression was restricted to the anterior and posterior edges of the autopod at HH stage 31 (I). Mouse Sp9 was detected in the AER and the surface ectoderm at E10.5 (J), E11.5 (K) and E13.0 (L). Zebrafish sp9 expression at 12 hpf (M) and 16 hpf (N). sp9 expression in the pectoral fin (arrowhead) at 36 hpf (O,Q) and 48 hpf (P,R,S). (S) An 8 µm transverse section of the pectoral fin bud of the embryo in P, showing the sp9 expression in the ectoderm. d, dorsal side; mhb, midbrain/hindbrain boundary; v, ventral side.





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