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Fig. 2. The onset of Gata2 expression in sympathetic ganglia in relation to the expression of Phox2b, Hand2, Phox2a and Th. Frozen sections from the brachial region of stage 18/19 chick embryos, staged according to the number of somites, were analysed for the expression of Phox2b, Hand2, Phox2a, Gata2 and Th. Gata2 expression was first observed in 34-somite embryos, after the onset of expression of Phox2b, Hand2 and Phox2a, but before Th, which was first observed in 35-somite embryos. Black and white arrowheads indicate the presence or absence, respectively, of gene expression in primary sympathetic ganglia. Gata2 expression was additionally detected in the ventral part of the dorsal aorta (da) (I) and in the spinal cord (D,I,N). The results are summarised in Table 1.





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