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Fig. 2. Amn is expressed in kidney proximal tubule (KPT) and in the epithelium of the small intestine. (A) Hybridization of the antisense Amn cDNA probe to a sagittal paraffin section of an E16.5 wild-type mouse embryo. The plane of section is shown in the schematic diagram on the left. Amn expression was detected in the intestinal epithelium and kidney. (B) In situ hybridization to adult kidney. Amn expression is restricted to the cortex and outer stripe of the outer medulla of the kidney. (C) The Amn protein is localized to the apical surface of proximal tubule (PT) cells. (D) Bright-field and (E) corresponding dark-field image of the adult small intestine. Amn expression is associated with intestinal villi. (F) The Amn protein is localized to the apical surface of the small intestine. (Inset) Staining with the preimmune antisera.





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