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Fig. 6. Adult Amn-/-{leftrightarrow}+/+ chimeras excrete increased levels of Cubn-specific ligands in the urine. Western blotting analyses of urine samples normalized for creatinine from adult chimeric females (A) and males (B). Loading volumes were equivalent to 163 ng creatinine (albumin) or 2.45 µg creatinine (transferrin and lysozyme). Estimated percent chimerism is indicated by the triangle above the figures and ranged from ~0% to 70%. C57/BL6 (B6) and 129 SvJ (129) mice were used as controls. While albumin is a ligand for both megalin and Cubn, transferrin is a Cubn-specific ligand and lysozyme is a megalin-specific ligand. (C) Albumin excretion by female chimeras and controls was quantitated by an ELISA assay and normalized by creatinine levels. Adult Amn-/-{leftrightarrow}+/+ chimeras excrete elevated levels of albumin in the urine when compared with controls. (D) Transferrin excretion, which was quantitated by an ELISA assay and normalized by creatinine levels, was elevated in adult female Amn-/-{leftrightarrow}+/+ chimeras when compared with female controls.





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