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Fig. 3. Masculinization of ATX-2-depleted germlines is caused by continuous
repression of tra-2 mRNA translation. (A-C)
tra-2(gf);atx-2(RNAi) gonad after staining with DAPI (A,B)
and mAb1CB4 (C). Sperm are absent (C, compare with
Fig. 1E) and the oocyte-like
germ cells are present (boxed region in A, magnified in B). Each arrowhead in
B, and in the wild-type control shown in D, indicates a single oocyte nucleus
with multiple highly condensed chromosomes (irregular bar-like objects). (E,F)
gld-1;atx-2(RNAi) gonad showing the presence of sperm. Boxed area in
E is shown at higher magnification in F; sperm nuclei (arrowheads) are small
and highly condensed. Some gld-1;atx-2(RNAi) germlines showed mitotic
proliferation proximal to the sperm; this phenotype was also observed in male
or masculinized hermaphrodite germlines depleted of GLD-1 at 25°C. (G-I)
Nomarski micrographs of fog-2 and fog-2;atx-2(RNAi) animals
with the gonads outlined in black. The gonads of most
fog-2;atx-2(RNAi) animals, as shown here, were small and contained
only oocytes similar to those in fog-2 gonads (arrows). Boxed area in
H is shown at higher magnification in I; the spermatheca (arrowhead) does not
contain any sperm. A minority of fog-2;atx-2(RNAi) animals contained
gonads with either both sperm and oocytes, or only sperm. Scale bars: 50
µm.
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