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Fig. 2. Embryonic Fpn1 mRNA expression is decreased in Pcm mutant mice. (A) Real-time RT-PCR analysis of E15.5 spleen shows reduction of Fpn1 mRNA in Pcm homozygous mutants. **, P<0.01. Decreased mRNA levels in placenta (B) and liver (C) in E16.5 Pcm mutants. *, P<0.05; **, P<0.01; ***, P<0.001. 5'RACE PCR of E15.5 spleen (D) and E16.5 placenta mRNA (E) demonstrates aberrant transcripts in Pcm homozygous mutants. Wild-type band, 630 bp.





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