|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 4. Anatomy of 
N-Zfp36l2 mutant female reproductive tract. Ovaries from
adult (8-12 weeks old) female mice, synchronized with respect to the ovulatory
cycle, were dissected from the fat pad, weighed and then fixed. The top and
middle panels show the macroscopic anatomy of the female reproductive tract
and the ovaries from wild-type (WT) (A,C) and N-Zfp36l2 mutant mice
(B,D). The lower panel shows representative ovary sections stained with
hematoxylin and eosin from females in post-estrus. No apparent abnormalities
were noted in the N-Zfp36l2 mutant ovaries (F) in comparison with the
WT (E); both exhibited follicles at various stages of development, as well as
corpora lutea (CL). Scale bars: 1 mm. (G) Western blot analysis was performed
using protein extracts from ovaries of WT (lanes 1) and N-Zfp36l2
mutant mice (lanes 2). These were probed with a carboxyl-terminal peptide
antiserum (AS) or with pre-immune serum (PI). The band corresponding to the
top band of Zfp36l2 was smaller in size in the ovaries from the mutant mice
(arrow), and the level of expression of the protein appeared to be decreased
in comparison with the WT. The smaller bands probably represent some
combination of degraded fragments of Zfp36l2, phosphorylated isoforms, or
possibly cross-reaction with the much smaller Zfp36l1 protein. In the presence
of competing peptide (middle panel), the AS recognition of the Zfp36l2 band
and the smaller bands was blocked.
|
