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Fig. 6. Fertilization of ova in 
N-Zfp36l2 mice. Ova from wild-type (WT)
(A-C, upper panels) and N-Zfp36l2 mutant females (D-F, lower panels)
were collected from the oviduct 8-10 hours after in vivo fertilization of
superovulated females, kept in culture for 4-5 hours, then fixed and stained
with a chromosome dye (Hoechst 33258) and analyzed by confocal microscopy.
These images are representative of three separate experiments. (A,D)
Differential interference contrast images show apparently normal morphology,
in which both ova are surrounded by zona pelucida. Chromosome staining of the
same cells (C,F) demonstrated that ova from both the WT and N-Zfp36l2
mutant females showed typical markers of fertilization, such as the presence
of two pronuclei and the formation of a second polar body (large filled
arrow). A supernumerary sperm is indicated by the filled small arrow in E,F.
In the middle panels (B,E), the left and right images were superimposed
showing the localization of the second polar bodies (large filled arrows) and
the two pronuclei (large open arrows) in relation to the surrounding zona
pelucida, as well as the supernumerary sperm in E (small filled arrows).
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