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Fig. 7. Arrest of embryos from 
N-Zfp36l2 females at the two-cell stage.
Wild-type (WT) and N-Zfp36l2 mutant females were superovulated and
mated with WT stud males. Nineteen hours after the hCG injection, all
potential in vivo fertilized ova were collected and incubated in M16 growth
medium. After 5 hours in culture, the fertilized ova were sorted based on the
morphological detection of two pronuclei and the presence of a second polar
body, thus considered time zero for the fertilization. The unfixed embryos
were photographed at 50x using Nomarski optics. The upper panel
illustrates the normal development of embryos from WT females after 48 hours
(A) and 72 hours (B) in culture; the embryos derived from N-Zfp36l2
mutant females at equivalent incubation periods are shown in C,D. The graph
represents means ±s.d. results from three such experiments, in which
the means of the numbers of actively dividing embryos derived from WT (black
circles) and N-Zfp36l2 mutant females (white circles) were
significantly different at 48 and 72 hours (P<0.01, using
Student's t-test), but not at 24 hours.
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