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Fig. 5. Duplications and expansions of node and notochord in
Tcf3-/- embryos. (A-B'') Scanning electron microscopy
(SEM) images of the ventral surface of wild-type (WT; A,A') and
Tcf3-/- (KO; B-B'') embryos (anterior towards the
lower left-hand corner). Arrows: Green, nodes; blue, notochords; red,
endodermal strip. White line indicates notochordendoderm boundary in B and
B'. White boxes in A and B outline areas magnified in A' and
B', respectively. Note the club-like structure of the single node and
notochord in the wild-type embryo and the duplicated node and expanded
notochord in mutant embryo. (C-F') Whole-mount in situ hybridizations of
E8.5 wild-type and knockout embryos, probed with digoxigenin-labeled cRNAs for
brachyury or Foxa2 as indicated. Anterior is towards the top of each
image. The three embryos in each panel in D and F show different
representative aberrant patterns, reflective of the extent of node/notochord
multiplication. Opposing arrowheads indicate thickness of notochords (ncd);
arrows indicate splitting of the notochord, often seen in mutant embryos.
(G,G') Tcf3-/- embryo with a rostral extension
probed for brachyury expression. Anterior is leftwards for both images. The
primary primitive streak (1°PS) and a secondary primitive streak
(2°PS) are positive for brachyury expression. Emerging from the secondary
primitive streak are structures similar to a node (arrows) and notochord
(arrowheads).
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