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Fig. 1. Salivary gland cells fragment as they die. (A) Twelve hour apf salivary glands have large vacuoles (asterisks) and nuclei (arrows). (B) Vacuoles (asterisks) are smaller in salivary glands of 14 hour apf animals, and nuclei (arrows) begin to separate from the cytoplasm. (C-E) Salivary glands rapidly degenerate 14.5 hours to 15 hours apf. (C,D) Nuclei (arrows) and cytoplasm of salivary glands separate 14.5 hours apf, and fragments of cytoplasm (enclosed in circle) are present. (E) By 15 hours apf, fragments of cellular debris are scattered throughout the area that was occupied by salivary glands. All images are at the same magnification.





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