QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

Help viewing high resolution images Return to article

Fig. 8. Inhibition of caspases prevents protein changes and salivary gland cell death. (A) The TUNEL assay was used to distinguish nuclei that contain fragmented DNA from nuclei with intact DNA. Animals staged 24 hours apf that express the dominant-negative form of Dronc C318A possess persistent salivary glands (SG) that lack vacuoles, but have nuclei with intact DNA (arrows), even though the DNA of the larval midgut (MG) is fragmented (dark stain, arrows). The brain (Br) is indicated to assist with orientation. (B) Expression of p35 and dominant-negative Dronc C318A prevent changes in the expression of -Tubulin (red) and -Spectrin (green), shown in the left panels, and nuclear Lamin DmO (red) (middle panels). By contrast, expression of p35 and dominant-negative Dronc C318A did not prevent the increase in abundance of Croquemort (green in the middle panels) in the cytoplasm, even though nuclear Lamin DmO (red) is expressed in the same cell, indicating that some cytoplasmic changes are not regulated by caspases. Similarly, filamentous Actin (red in the right panels) changes proceed and resemble 12-hour apf wild-type salivary gland cells, even though expression of p35 and dominant-negative Dronc C318A prevent the death of these cells.

Return to article