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Fig. 9. Distal growth plate abnormalities in radius and ulna of mice with three
mutant alleles of Hoxa11 and Hoxd11. (A) Forelimb skeleton
of control newborn
(Hoxa11+/+Hoxd11+/-) stained with
Alcian Blue and Alizarin Red. (B) Forelimb skeleton of
Hoxa11+/-Hoxd11-/- newborn. (C)
Hematoxylin and Eosin-stained section of 14-day-old control forelimb showing
growth plate of the distal ulna. (D) Growth plate of distal ulna in 14-day-old
Hoxa11-/- Hoxd11+/- mouse. Asterisks
are adjacent to secondary ossification centers. (E) Distal growth plate of
ulna in newborn (P0) control specimen. (F) Growth plate of distal ulna from a
P0 Hoxa11+/- Hoxd11-/- mouse.
Double-headed arrows demarcate proximodistal extent of growth plates. (G,H)
Bright-field views of distal radius and ulna of Hoxa11-/-
Hoxd11+/- newborn (hz, hypertrophic zone). (I) Dark-field
image of G, showing hybridization with the Ihh probe. (J) Dark-field
image of H, showing Pthr expression. (K) Hoxd11 expression
pattern at E16.5 revealed by in situ hybridization with a
33P-labeled probe. Transcripts are detectable adjacent to the
distal end of the ulna (white bracket).
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