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Fig. 3. Morphology and gene expression in Gli2 null brains. (A) Dorsal
view of the morphology of wild-type (left) and Gli2-/-
(right) dissected E18.5 brains. Anterior is to the top. (B,C) Comparison of
lateral views of dissected cortex, and dorsal views of tectum and cerebellum
in wild-type (B) versus Gli2-/- (C) brains. Arrows point
to the posterior cortex in the two hemispheres, tectum and cerebellum.
Gli2-/- mutant mice have an apparently normal choroid
plexus (not shown). The ballooning of the telencephalic vesicle may be due to
the inability of the tissue, which is thinner than normal, to sustain the same
degree of intravesicular pressure. (D) Comparison of wild-type and
Gli2-/- cortices seen in parietal sagittal sections
stained for Hematoxylin and Eosin. The intermediate zone and cortical plate
layers appear of normal size and cell density. (E-G) BrdU incorporation in
wild-type (F) and mutant (G) cortices, and quantification of cell
proliferation (E). The mean number of BrdU+ cells per section from
wild-type and Gli2-/- animals is shown. For simplicity,
the vz was considered as the zone in between the ventricle and 
5-cell
diameters away, and the svz as that in between 5- and 10-cell diameters
from the ventricle (svz cells, P<0.05; vz cells,
P<0.01). (H,I) Comparison of Neurod1 expression by in
situ hybridization in the cerebellum of wild-type (H) and
Gli2-/- (I) E18.5 animals. (J-S) Images of in situ
hybridization analyses of sagittal sections from E18.5 (J-Q), and coronal
sections from E15.5 (R,S), wild type (J,L,N,P,R) and
Gli2-/- (K,M,O,Q,S) animals probed with Gli1
(J,K), Gli2 (L,M), Gli3 (N,O), Neurod1 (P,Q) or
clone 53 (R,S). Neocortical Gli1 expression is absent in
Gli2-/- animals, but a domain of its expression in the
striatum was still present (inset, K). Note the small hippocampus in the
Gli2-/- brain (Q, arrow), compared with in wild type (P).
Forebrain pattern appeared largely unchanged as expression of the
Pax6 anteroposterior gradient and of Dlx2 ventrally were not
grossly affected (not shown). (T,U) Quantification of the number of
Neurod1+ (T) and clone 53+ (U) cells in the
dorsal telencephalon of wild-type versus Gli2-/- animals
(Neurod1+, P<0.001; clone 53+,
P<0.001). cb, cerebellum; cp, cortical plate; ctx, cortex; h,
hippocampus; iz, intermediate zone; Med, medulla; st, striatum; svz,
subventricular zone; tct, tectum; vz, ventricular zone. Scale bar in A: 800
µm for A; 1.3 mm for B,C; 75 µm for D; 50 µm for F,G,H,I; 130 µm
for J-Q; 300 µm for R,S.
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