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Fig. 4. The Notch target gene Hairy2A produces an expansion of the neural
crest population. Eight-cell embryos were injected in two blastomeres with 1
ng Hairy2A mRNA, the arrowhead indicates the injected side, which was
visualized by alkaline phosphatase-mediated FITC inmunodetection. (A)
N-tubulin expression is clearly reduced. (B) Immunodetection of the
somite antigen 12/101 analyzed at stage 25. Note that there is no difference
in the staining between the injected and uninjected side. (C) The domain of
Xslug expression is expanded on the injected side, whereas
Bmp4 is dramatically repressed (D,E). (E) Corresponds to a higher
magnification of D. (F) Xmsx1 expression is increased on the injected
side. Each experiment was performed at least twice with a minimum of 35
embryos. The effect seen in each experiment was observed in at least 70% of
embryos.
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