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Files in this Data Supplement:
Movie 1. Time-lapse recording of the embryo shown in Fig. 4.
Movie 2. Time-lapse recording of the formation of the venous plexus dorsal to the vitelline artery. Recording started at the 19 ss and lasted for a period of 20 hours. Pictures were taken every 10 minutes. The movie starts with the arterial plexus, veins are not yet present. Note appearance of blood-filled ‘spots’ during development of the vitelline artery and reconnection to the forming lateral vitelline vein. At the end of the movie, veins cross arteries dorsally.
Movie 3. High-magnification intravital video-recording of the formation of the lateral vitelline vein, corresponding to Fig. 5A-J. Note that all blood flow recorded in sequence 1 between the two branches of the vitelline artery (A) is forward. Yellow arrow indicates the blood-filled ‘spots’. Note the progression of the blood-filled ‘spots’ (sequence 1-3). Green arrows in sequence 3 indicate the onset of sprouting. In sequence 4, reconnection to the lateral vitelline vein (V) has been achieved. High-magnification zoom shows small sprouts crossing the vitelline artery, blood flow in the sprouts is now reversed, showing their reconnection to the venous system (V).
Movie 4. Time-lapse recording of the same embryo shown in Fig. 8 over a period of 20 hours. Ligation results in venularization of the arterial tree.
Movie 5. Time lapse recording of the anterior vitelline vein after ligation of the right vitelline artery. Integration of the anterior vitelline vein into the left vitelline artery while it crosses the midline.
Movie 6. Time-lapse recording of the embryo shown in Fig. 10A. Reversal of flow in the vitelline artery caused by retrograde perfusion from the peripheral sinus vein.
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