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Fig. 6. Mercox casts and QH1 whole-mount staining of the developing vitelline artery. (A,B) Scanning electron micrographs of mercox-filled vitelline artery (VA). (C,D) QH1 staining. QH1 endothelial surface staining shows the same morphology as the mercox cast of the vessel lumen. (A) Asterisks show capillary free-zones. Arrowheads indicate disconnected arterial capillaries that are perfused from distal parts and that correspond to the blood filled `spots' in Fig. 5. (B,D) Higher magnification of the boxed areas in A, C, respectively. Blind ending sacs (B,D, arrows) are present on both sides of the vitelline artery and there are corresponding protrusions on opposite capillary segments (B, double arrows). Note the presence of a capillary sprout (Sp) dorsal to the vitelline artery (VA). Scale bars: 160 µm in A; 70 µm in B; 125 µm in C; 20 µm in D.





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