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Fig. 7. No-flow and incision models. (A) Bright field photomicrograph of a no-flow yolk sac of an E5 quail embryo. Note the absence of arteries and veins. (B,C) Whole-mount in situ hybridization of an E5 quail embryo no-flow yolk sac with an ephrinB2 anti-sense riboprobe. Part of the capillary plexus expresses ephrinB2 (B), other parts are ephrinB2 negative (C). (D) Incision model. 35 ss embryo 24 hours after incision. The incision site separating the embryo from the left side of the yolk sac is indicated (stippled area). A single vitelline vein (VV) has developed on the left side. The left vitelline artery (VA) is absent. The right VA shows increased growth. Asterisk indicates the heart. Scale bars: 300 µm in A; 50 µm in B,C; 600 µm in D.





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