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Fig. 4. Gurken and yolkless trafficking are disrupted in sec5^E13 oocytes. (A-G,J-M) Egg chambers, genotype indicated, were stained with phalloidin (red), Hoechst (blue), and anti-Gurken (green, A-G) or anti-Yolkless (green, J-M). (A,D) At early stages, Gurken accumulates in the oocyte in both wild-type and sec5^E13germlines. (B,E) At stage 8, Gurken is mislocalized within the cytoplasm of sec5^E13 oocytes. (C,F) Gurken localizes at the anterodorsal membrane in stage 10 wild-type oocytes (arrow and enlargement), but in sec5^E13 mutant oocytes accumulates in puncta within the ooplasm. Little Gurken is observed in the membrane (arrow and enlargement). (G) A compressed z stack of a sec5^E13 stage 10 egg chamber, displaying mislocalized Gurken protein throughout the ooplasm. (H,I) Oocyte yolk granules visualized by Nomarski optics. (J,K) Yolkless (green) accumulates in the cytosol of stage 7 oocytes. (L,M) By stage 10, Yolkless shifts to the plasma membrane of wild-type but not sec5^E13 oocytes. Scale bars: 20 µm.

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