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Fig. 2. Metasynchronous mitoses occur prior to cycle 10. (A-F) are images from a
time-lapse recording of a histone-GFP embryo at cycle 7. All nuclei are at the
beginning of interphase (A) and then enter prophase (B). (C) The nucleus in
the posterior region enters anaphase (black arrow), while the one in the
middle (white arrow) remains in metaphase and enters anaphase 40 seconds later
(D). (E) Both nuclei are in telophase. The two daughter nuclei in (E) moved
out of the focal plane but all other nuclei show early interphase
configuration of cycle 8 at the same time (F). That nuclei in different
regions enter interphase at the same time is supported from other time-lapse
recordings. (G) Summary of the regional differences of cell-cycle-phase
durations in histone-GFP embryos at cycle 7 (n=9) and cycle 8
(n=17), showing that prophase-metaphase is longer and
anaphase-telophase is shorter in the middle region than in the posterior
region. The total cell cycle time at cycle 8 is not significantly different in
the two regions. (H) An epifluorescence image showing metasynchronous mitoses
at cycle 7 from a fixed wild-type embryo: nuclei at the two polar regions are
in anaphase, whereas the nuclei in the middle region are in metaphase. Scale
bars: 100 µm.
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