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Fig. 6. Cytoplasmic flow, as indicated by traces of particle movement, during axial
expansion (cycle 4 to cycle 8) may contribute to uneven distribution of CycB
within an embryo. Both A and B are projections of three-minute time-lapse
recordings every 10 seconds of a wild-type embryo at cycle 6. These
bright-field transmission images were recorded on a two-photon optical
workstation (Wokosin et al.,
2003). There is little particle movement in interphase [A, see
Baker et al. (Baker et al.,
1993) for staging]. By contrast, dramatic particle movement can be
observed between interphase and anaphase [B, see Baker et al.
(Baker et al., 1993) for
staging]. Note the inward flow in the middle region. (C) The direction of the
flow in prophase and metaphase. Scale bar: 50 µm.
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