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Fig. 3. Development of vertebral defects in Tsg mutant embryos.
(A-A') Hematoxylin and Eosin staining of sagittal sections of the tail
of E12.5 wild-type and Tsg-/- embryos. (B-B')
Mallory's tetrachrome staining of sagittal sections of E15.5 wild-type and
Tsg-/- tails. Vertebral bodies (vb); intervertebral
regions (iv) consisting of an outer annulus (oa) and inner annulus (ia);
nucleus pulposus (np) containing the remnants of the notochord. In the wild
type, the np is surrounded by the prechondrogenic mesenchyme of the ia.
(B') The Tsg mutant vertebral bodies are more rectangular and
narrower than wild type; the intervertebral region is expanded, notochordal
cells are not resorbed in the vertebral bodies. (C-C') Alcian Blue
staining of E14.5 wild-type and Tsg-/- embryos. Note the
smaller vertebral bodies and persistence of the notochord (n) in the mutant.
(D-D') ß-gal staining of the tail region of E14.5 wild-type and
Tsg-/- embryos showing expanded Tsg expression in
intervertebral prechondrogenic mesenchyme and in the notochord of the mutant.
(E-E') Expression of Bmp4 at E14.5 in wild-type and
Tsg-/- embryos. (E) Bmp4 is expressed in the
intervertebral annulus fibrosus, which is expanded in the mutant (E').
cm, condensed mesenchyme; scl, sclerotome; s, somite; um; uncondensed
mesenchyme; sp, spinal chord.
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