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Fig. 6. Comparative expression of Tsg, Bmp4 and Chd. Whole-mount in situ hybridization was performed between E8.0 and E10.0 using the gene markers indicated. (A) Tsg expression at E8.0, frontal view. (B,C) Frontal and lateral views of Bmp4 expression at E8.0. Inset shows expression in the surface ectoderm (se) at E7.75. (D) Chd expression at E8.0 in the node (no), notochord, prechordal plate and endoderm. (E) ß-Gal staining showing diffuse expression of Tsg at E9.0. (F) Expression of Bmp4 at E9.0. Inset shows expression in the branchial arch as early as E8.5. (G) Expression of Tsg at E10.0. (H) Expression of Bmp4 at E10.0. a, allantois; a1, first branchial arch; aip, anterior intestinal portal; bd, basal diencephalon; bm, basal mesencephalon; bt, basal telencephalon; dt, dorsal telencephalon; fn, frontonasal process; fp, floor plate; h, hyoid arch; lb, limb bud; m, mesoderm; md, mandibular; mx, maxillary; nf, neural fold; vm, ventral mesoderm.





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