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Fig. 2. Scheme of map-based cloning and the structure of the RBE gene. (A) The genomic structure around RBE on chromosome 5. Numerals indicate the number of recombinants. Arrows indicate the open reading frames around the RBE gene. (B) cDNA and predicted amino acid sequences of RBE (GenBank accession no. AB107371; the locus name is At5g06070). The zinc finger motif is underlined, and the QALGGH motif specific to EPF zinc finger proteins in plants is boxed. The Potential nuclear localization signal is shown in bold type. The broken line indicates the leucine-rich domain of the carboxyl terminus. Mutations of rbe-1 and rbe-2 are shown. (C,D) Sequence alignment of the zinc finger domain (C) and the carboxy-terminal leucine-rich motif (D) in single zinc finger genes belonging to the RBE-SUP family in Arabidopsis thaliana. Asterisks in C indicate the conserved cysteine and histidine in the zinc finger domain. Consensus sequences are shown below the alignments.





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