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Fig. 1. DAF-5 encodes a C. elegans homolog of the Sno and Ski
oncoproteins. (A) Identification of daf-5-coding region. The genetic
map near daf-5 and rescued transgenic lines isolated are shown. (B)
DAF-5 gene structure. At the C terminus of DAF-5, a stop codon and a deletion
mutant are connected by a broken line to indicate that these two mutations
were identified in a single allele. A mutation hotspot is shown; an additional
mutant has an in-frame deletion of the 15 amino acids shown. Colors of amino
acids in hotspot represent sequence conservation as in Fig. 1C,D. See Table S2
at
http://dev.biologists.org/supplemental
for complete details on mutant alleles. (C,D) Alignment of SDS and Dach boxes.
Consensus at each position was defined as any set of identical and similar
amino acids that were found in more than one subgroup (subgroups are indicated
by spaces between the rows of sequence), and in more than three proteins in
total. Bold magenta residues are identical in the primary consensus (the
consensus with the most matches) and plain, magenta are similar. Green
residues are the secondary consensus. The % symbols below the SDS box show
conserved zinc chelating residues, and the asterisks indicate amino acids that
contact Smad4 in the crystal structure of a Ski/Smad4 complex. The sequence
for Dog Nem. is from an EST, and has two stop codons in frame (indicated by
X). These may result from sequencing errors, or the cDNA may have come from a
pseudogene. Accession Numbers and abbreviations: Dros., D.
melanogaster (Iceskate: NP_651946; Snowski: NP_609166; Dachshund:
NP_723972); Mosq., mosquito (A. gambiae; Iceskate-XP_317739;
Snowski-XP_317545); Soy. Nem., Soybean Cyst nematode (Heterodera
glycines; CA939358); Root nem., Root Knot nematode (Meloidogyne
chitwoodi; CB931358); Pot. Nem., Potato Cyst nematode (Globodera
rostochiensis; AY389814); Lymph Nem, Brugia malayi (AY389813);
Dog Nem, dog hookworm (Ancylostoma caninum; AW735310); C.
briggsae DAF-5 (found in Wormbase as CBG20832); C. elegans
(DAF-5-NM_064540, NP_496941; Dachshund-NP_497266); Human (Dachshund-NP_542937;
cSki-NP_003027; SnoN-NP_005405; Icy-XP_292349; Skate-XP_064560); sea squirt
(Ciona intestinalis; Ski-BK001616; Dachshund-AABS01000073).
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