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Fig. 4. daf-5 controls cell cycle arrest in the seam cells of dauer larvae
by regulating expression of a cyclin kinase inhibitor. (A,B)
rnr-1::GFP expression in N2 and daf-5; daf-1 double mutants.
A corresponding Nomarski image can be found in Fig. S2 at
http://dev.biologists.org/supplemental.
White arrowheads show seam cells that express rnr-1::GFP in N2 and in
daf-5; daf-1, respectively, at early L3 stage. (C,D)
cki-1::GFP expression in daf-1 and daf-5; daf-1
mutants. A corresponding Nomarski image can be found in Fig. S2 at
http://dev.biologists.org/supplemental.
(C) cki-1 is expressed along the seam cells in all daf-1
dauer larvae with an average of 16 green cells. White arrowheads (E) show seam
cell nuclei. (D) cki-1 is expressed in a subset of daf-5;
daf-1 mutants in early L3. Two daf-5; daf-1 animals are shown.
The top animal is representative of the 37% of animals of this genotype that
had no GFP in the seam at all. The bottom animal is representative of the 34%
that expressed GFP faintly in many seam cells (between five and ten). White
arrows point to the seam cells that do not express cki-1::GFP in the
nuclei. Scale bars: 1 µm.
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