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Fig. 3. (A) Western transfer analysis of Dd-STATb. Cells were allowed to develop on water agar, aliquots were harvested at the indicated times and they were subjected to western transfer analysis using the C:STATb antibody. This result, combined with other gel analyses using high molecular weight markers (B), show that Dd-STATb migrates with the expected approximate molecular weight of 130 kDa. (B) Western transfer analysis of two Dd-STATb disruptant clones and two random integrant clones. The four clones were grown to 2x106/ml and subjected to western transfer using the C:STATb antibody. Clones B15 and B16 are random integrant (Dd-STATb+) clones and B8 and B9 are disruptant (Dd-STATb-) clones. These assignments were further confirmed by immunohistochemical staining (data not shown). (C) Immunohistochemical analysis of the intracellular distribution of Dd-STATb in growing cells. Cells growing at 2x106/ml in HL5 medium were fixed and stained with the C:STATb antibody. Scale bar: 10 µm.





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