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Fig. 8. Biochemical analysis of potential interactions between Dd-STATb and other
STATs. (A) Growing cells were lysed and subjected to immunoprecipitation using
the C:STATb antibody and the pellet was assayed for the three STATs using the
analysis protocol described on the figure. (B) Genetic analysis of potential
interactions between Dd-STATb and other STATs. Slugs were generated using
either Ax-2 cells or cells that are null for both the Dd-STATa and the
Dd-STATc genes. The latter strain was created by sequential inactivation,
using ura and blasticidin disruption cassettes (Kawata et al., 1996;
Kalpaxis et al., 1991).
Absence of both STAT proteins in the selected strain was confirmed
immunochemically. Whole-mount slugs were fixed and stained using the C:STATb
antibody and visualised by confocal microscopy. Only the front approximate
half of each slug is shown and their tips are facing left.
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