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Fig. 9. Glycerol gradient analysis of unmutated and mutated forms of Dd-STATb. Two
mutant versions of Dd-STATb, and the unmutated Dd-STATb, were cloned
downstream of the constitutive actin 15 promoter; in LR residue L1025 is
converted to arginine and in YF residue Y1143 is converted to phenylalanine.
These three DNAs were stably transformed into Dictyostelium using
G418 as the selective agent. This yields multiple copies of the transforming
DNA, the number varying from cell. Clones with a high expression level were
selected and analysed as described in the legend to
Fig. 7. Scale bar: 10
µm.
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