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Fig. 2. Analysis of differentiation marker expression on the sorted cell subpopulations. RNA from whole testis cell suspension of mutant W/Wv males was analyzed in parallel with a sorted cell population (according to Fig. 1B) from normal adult males: population 1 (SP), population 3 (4n), upper diploid population 4 (2n) and population 5 (n). RT-PCR was performed for Kit (35 cycles), Dazl (30 cycles), H1t (30 cycles), Hsp70-2 (30 cycles) and Crem {tau} (30 cycles) genes. ß-actin (30 cycles) served as a normalization control.





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