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Fig. 3. Analysis of the SP population for the expression of {alpha}6-integrin and Stra8 germinal stem cell markers. Ho fluorescence flow cytometric analyses of {alpha}6-integrin-positive cell fraction (A) and {alpha}6-integrin-negative cell fraction (B) after immunomagnetic sorting. (C) RT-PCR analysis of Stra8 gene expression (35 cycles) on W/Wv, and sorted normal populations: SP cells (SP), tetraploid population (4n), upper diploid population (2n) and haploid population (n).





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