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Fig. 2. The anterior part of a female ovariole and male testis in Drosophila
melanogaster. Anterior is up. (A) An ovariole from a female
Drosophila carrying a ß-galactosidase (ß-gal) enhancer trap
in the dad gene, stained with antibodies against ß-gal (green),
and with the monoclonal antibody 1B1 (red), which stains the membrane
cytoskeleton in somatic cells and the fusome within germ cells. The terminal
filament and cap cells are at the anterior and are marked with brackets. GSCs
are located just posterior to cap cells, and an arrowhead marks a spherical
fusome in one GSC that is abutting cap cells. An arrow marks a branched fusome
within a cyst in a more posterior location within the germarium. ß-gal
staining is strong in GSCs, but can also be observed in their immediate
daughters and early cysts. This may be due to the produrence of the ß-gal
protein. (B) A testis from a male Drosophila carrying a ß-gal
enhancer trap in the escargot gene, stained with anti-ß-gal
(green) and 1B1 (red) antibodies. Hub cells express ß-gal most strongly
and are circled. Arranged around the hub are GSCs, which also express
ß-gal. Staining of ß-gal in gonialblasts and early cysts may be due
to the produrence of the protein. Posterior to the hub are the dividing cysts;
a fusome in one cyst is marked by an arrow. (B inset) The same genotype as in
B, stained with anti-ß-gal (green) and with anti-Fasciclin III (red),
which outlines the cells of the hub, showing its compact structure. Scale bar:
20 µm.
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