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Fig. 1. Eda-A1 overexpression leads to increased size and irregular shape of the
early hair placodes. Placodes were larger in K14-Eda-A1 transgenic
embryonic E14 skin than in wild-type skin, as visualised by whole-mount in
situ hybridisation by marker genes ß-catenin (A,B) and Edar
(C,D). E-H are higher magnification views of A-D. Fusions (arrow in B and F)
and the irregular shape (arrows in D and H) of the early hair follicles were
common in transgenic skin. In radioactive in situ hybridisation analysis, the
expression of Lef1 was seen throughout wild-type (I) and transgenic
(J) skin. Placodal epithelial and mesenchymal marker Ptch1 also
showed expansion of the hair follicles in the transgenic skin (arrow in L)
when compared with wild type (K). Expression of Bmp4 mRNA localised
to the mesenchyme under the forming follicles in wild-type (M) and transgenic
(N) skin. Enlarged hair follicles with irregular shape in the transgenic
ectoderm (P) were evident in thin plastic sections of the E14 skin, whereas
wild-type follicles were smaller and had a regular round shape (O). Scanning
electron microscopy images also revealed irregularities in shape and increased
sizes of the hair placodes of the transgenic embryos (R) and fusions of their
follicles (T), when compared with the wild-type embryos with smaller regular
placodes (Q,S).
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